FastQCFastQC Report
Mon 23 Dec 2019
H2CVWAFX2_n01_AH9003-3h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVWAFX2_n01_AH9003-3h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11587401
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1887951.629312733718286TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA354650.063.3593839
AGAGCAC365650.061.5580258
GATCGGA402300.054.9187361
TCGGAAG412400.054.4970323
CGGAAGA426550.052.88974
GAAGAGC433900.052.0813876
ATCGGAA435100.051.5461732
AAGAGCA450150.050.1828887
GGAAGAG458650.049.28135
GCCGTCT244500.042.0204748-49
TGCCGTC255100.041.33574348-49
GTATGCC264300.040.7856944-45
ACTGACC269850.040.37730832-33
CGTATGC268350.040.20554744-45
TATGCCG270150.039.657246-47
ATGCCGT269350.039.325346-47
CTCGTAT254500.039.25778642-43
TCGTATG275450.038.9102142-43
CAGTCAC290850.038.6621826-27
ATCTCGT254650.038.4884140-41
Produced by FastQC (version 0.11.5)