FastQCFastQC Report
Mon 23 Dec 2019
H2CVWAFX2_n01_AH9003-3h-Red1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVWAFX2_n01_AH9003-3h-Red1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11113375
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT1705241.5344033653143172TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA390600.053.819679
AGAGCAC407650.051.6271488
TCGGAAG463950.045.2153173
CGGAAGA467100.045.0528074
GAAGAGC480000.043.8816076
GTCCCGA225200.043.6275436-37
CGTCCCG230600.043.55319634-35
GATCGGA480850.043.1999471
ATCGGAA490900.042.714832
AAGAGCA498750.042.3317
CCCGATC218050.042.21562638-39
TGCCGTC239750.041.86260250-51
GGAAGAG503150.041.8248375
ACCCGTC243600.041.7357932-33
CGATCTC220450.041.51902840-41
GCCGTCT235900.041.3779750-51
CCGTCCC247150.041.14600834-35
CCGATCT220350.041.03130738-39
CCCGTCC247100.040.89474532-33
TCACCCG251000.040.76070430-31
Produced by FastQC (version 0.11.5)