FastQCFastQC Report
Mon 23 Dec 2019
H2CVWAFX2_n01_AH8632-3h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVWAFX2_n01_AH8632-3h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12075904
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC2172201.7987887283635244TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC310050.25675096456546853TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTTTGC122720.10162386186574521TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA605600.068.624939
AGAGCAC618700.067.264028
GATCGGA653150.062.6786581
TCGGAAG663300.062.5745663
CGGAAGA676650.061.487474
GAAGAGC687400.060.6364946
ATCGGAA685500.060.5136762
AAGAGCA709650.058.8453527
GGAAGAG712950.058.3834765
TGCCGTC271900.041.6467448-49
CCGATGT490150.041.2764132-33
TATCTCG345950.041.24450338-39
GCCGTCT238500.041.1458448-49
TATGCCG309000.041.12681646-47
GTATGCC318850.041.01775444-45
CGATGTA493350.040.93689734-35
ACCGATG503500.040.4838632-33
CGTATGC317150.040.40642544-45
CACCGAT507100.040.33210830-31
GTCACCG510200.040.24514428-29
Produced by FastQC (version 0.11.5)