FastQCFastQC Report
Mon 23 Dec 2019
H2CVWAFX2_n01_AH8632-3h-Top2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVWAFX2_n01_AH8632-3h-Top2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11692920
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1576601.3483372844422095TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGAT465510.3981127040978643TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTTT339450.2903038761917468TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGTAT314250.26875237323098083TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGAT291590.24937312493372057TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGTT291060.2489198591968473TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGTT264740.2264105116600473TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGGGGTT181290.15504253856179637TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGGGGGT168270.1439075953654006TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGTTT153790.13152403334667473TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA731950.077.5816969
AGAGCAC741450.076.677678
GATCGGA760650.073.308431
TCGGAAG773800.073.1408543
CGGAAGA795200.071.357764
ATCGGAA799200.070.877282
GAAGAGC803350.070.793536
GGAAGAG825750.068.769555
AAGAGCA828900.068.7267767
AGTCACA661900.042.774928-29
ATGTCAG645500.042.76898234-35
TCACATG653750.042.7668930-31
CAGTCAC658450.042.72824526-27
CCAGTCA663250.042.68039726-27
CACATGT649050.042.6045830-31
ACATGTC651900.042.57860632-33
GTCACAT655550.042.42848228-29
TGTCAGA642050.042.3034234-35
TCCAGTC663450.042.25561524-25
GAACTCC672400.041.87700720-21
Produced by FastQC (version 0.11.5)