FastQCFastQC Report
Mon 23 Dec 2019
H2CVWAFX2_n01_AH7838-3h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVWAFX2_n01_AH7838-3h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12658168
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1893831.4961327737157541TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA367500.059.3471649
AGAGCAC384700.056.8297738
GATCGGA413300.051.754471
TCGGAAG422600.051.405013
ATCGGAA441050.049.2771572
CGGAAGA444500.049.085944
GAAGAGC453950.048.451386
GGAAGAG475200.046.0847555
AAGAGCA478050.046.031827
GCCGTCT241650.040.6785850-51
TGCCGTC249900.040.4000650-51
GTATGCC256850.039.79617346-47
TATGCCG263700.038.8169848-49
CGTATGC265550.038.73383346-47
CTCGTAT260150.038.3869844-45
AGGGGGG217250.038.10943274-75
ATGCCGT266250.037.9189348-49
CACAGTC279800.037.85481630-31
ATCTCGT265650.037.8244342-43
TCTCGTA267550.037.5469442-43
Produced by FastQC (version 0.11.5)