FastQCFastQC Report
Mon 23 Dec 2019
H2CVWAFX2_n01_AH7838-3h-Red1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVWAFX2_n01_AH7838-3h-Red1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11976120
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC631430.5272408760099264TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC184650.1541818218254326TruSeq Adapter, Index 8 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA357250.072.297169
AGAGCAC372750.069.507748
TCGGAAG390300.065.9775853
GATCGGA385000.065.780441
ATCGGAA404000.063.776842
CGGAAGA406400.063.6441234
GAAGAGC441100.058.7559176
GGAAGAG460150.056.2614945
AAGAGCA467300.055.5974467
GTCACAC305000.041.789328-29
CAGTCAC318600.040.51960426-27
ACGTCTG325800.039.76197414-15
GAACTCC325550.039.73514620-21
TCCAGTC324750.039.71552724-25
AGTCACA326150.039.36334628-29
CCAGTCA327400.039.34345626-27
ACACGTC330250.039.28372212-13
TCACACT323950.039.18348330-31
CACGTCT331600.039.02353314-15
GCACACG331850.038.97981610-11
Produced by FastQC (version 0.11.5)