FastQCFastQC Report
Mon 23 Dec 2019
H2CVWAFX2_n01_AH7797-4h-inputTop2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVWAFX2_n01_AH7797-4h-inputTop2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11707040
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC3121112.6660112205988873TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA633450.055.3724029
AGAGCAC653400.053.6817478
GAAGAGC734950.047.8136636
TCGGAAG740500.047.2886853
CGGAAGA742150.047.2603724
AAGAGCA745250.047.244677
GGAAGAG769100.045.6720855
GATCGGA755500.045.5993461
ATCGGAA774450.045.1063842
GCCGTCT357400.043.0866248-49
TGCCGTC371350.042.9262148-49
GTATGCC386450.042.29818744-45
TATGCCG386400.042.2002646-47
CGTATGC391500.041.84356744-45
ATGCCGT381950.041.7716846-47
TATCTCG398900.041.43093538-39
CTCGTAT394800.041.15651742-43
CCGTCTT380850.040.75177850-51
ATCTCGT408250.040.5339840-41
TCGTATG402850.040.4284242-43
Produced by FastQC (version 0.11.5)