FastQCFastQC Report
Mon 23 Dec 2019
H2CVWAFX2_n01_AH7797-4h-Top2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVWAFX2_n01_AH7797-4h-Top2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11580861
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT8937407.717388197647826TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTAT185720.16036804171986868TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGCTCTCGTAT156130.13481726445037204TruSeq Adapter, Index 19 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG142510.12305648086096534No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAGCTCGTAT139510.12046599989413567TruSeq Adapter, Index 19 (97% over 40bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG117640.1015813936459474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1226350.082.386439
AGAGCAC1241650.081.467228
TCGGAAG1278150.078.820023
GATCGGA1270150.078.234621
GAAGAGC1301250.077.734796
CGGAAGA1302000.077.649884
ATCGGAA1303950.077.2906342
AAGAGCA1324200.076.559487
GGAAGAG1328650.076.053045
TGCCGTC1083750.045.50838550-51
CGATCTC1005450.045.45726440-41
GTATGCC1087850.045.3583146-47
GCCGTCT1071850.045.32011850-51
CGTATGC1096650.045.2455446-47
AGTCACG1110150.045.23344828-29
TATGCCG1095150.045.17532748-49
GATCTCG1014650.045.07317740-41
CTCGTAT1057350.044.9931844-45
ATCTCGT1026350.044.93624542-43
CCGTCTT1091000.044.76833352-53
Produced by FastQC (version 0.11.5)