FastQCFastQC Report
Mon 23 Dec 2019
H2CVWAFX2_n01_AH7797-3h-inputNop1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVWAFX2_n01_AH7797-3h-inputNop1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10140521
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1720151.6963132367656457TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC1385121.3659258730394621TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGC246960.2435377827233926TruSeq Adapter, Index 5 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC139410.13747814338139036TruSeq Adapter, Index 5 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG119320.11766653804079691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA790000.067.625189
AGAGCAC809150.066.0896158
GAAGAGC879400.060.7938776
AAGAGCA889100.060.2437257
CGGAAGA890300.060.0922744
TCGGAAG888650.060.0594753
GATCGGA893350.058.79091
GGAAGAG908850.058.76125
ATCGGAA913950.058.392862
GTATGCC419000.043.50684744-45
TGCCGTC364850.043.40986348-49
TATGCCG411700.043.2750146-47
GCCGTCT322300.043.25335748-49
CGTATGC422900.042.99892844-45
CACACAG622550.042.34295730-31
CACAGTG618700.042.2302832-33
ATGCCGT382350.042.15583846-47
GTCACAC625150.042.10585828-29
AAGGGGG442450.042.05751872-73
ACACAGT631050.041.64842632-33
Produced by FastQC (version 0.11.5)