FastQCFastQC Report
Mon 23 Dec 2019
H2CVWAFX2_n01_AH7797-3h-Nop1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVWAFX2_n01_AH7797-3h-Nop1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11122362
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT2643522.3767613390033517TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA495150.063.0121429
AGAGCAC516700.060.5127838
TCGGAAG565150.055.4522633
CGGAAGA578000.054.3920824
GATCGGA570700.054.374781
ATCGGAA592550.052.8798522
GAAGAGC597500.052.545376
AAGAGCA607900.051.6074267
GGAAGAG620600.050.6124955
CGTCCGC351550.043.26867332-33
ACGTCCG352950.043.03648432-33
CATCTCG333300.042.6309740-41
GTATGCC347700.042.62846-47
TGCCGTC342200.042.45294650-51
CCGCACA349150.042.4439736-37
GTCCGCA357050.042.15024634-35
GCCGTCT335150.042.14131550-51
ATGCCGT349800.041.91050748-49
TCCGCAC360800.041.78455734-35
CGTATGC355150.041.69366546-47
Produced by FastQC (version 0.11.5)