FastQCFastQC Report
Mon 23 Dec 2019
H2CVWAFX2_n01_AH7797-3h-34C-input-spikerep.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVWAFX2_n01_AH7797-3h-34C-input-spikerep.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12740460
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1177230.9240090232220814TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC213740.16776474318823653TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA426900.042.8897679
AGAGCAC443600.041.2644168
GCCGTCT168850.038.2719248-49
TGCCGTC182000.038.05124748-49
GTATGCC200500.037.0269544-45
TATGCCG198600.036.7599446-47
CGTATGC203250.036.5610344-45
ATGCCGT196100.035.5451846-47
TCGGAAG523100.034.951523
CGGAAGA530700.034.585294
GAAGAGC531850.034.5373046
TCGTATG213450.034.19037242-43
AAGGGGG174850.034.1615172-73
GATCGGA533000.033.676951
AAGAGCA547650.033.659267
CTCGTAT185550.033.62300542-43
CCGTCTT200050.033.07504750-51
ATCTCGT195450.032.8556640-41
GGAAGAG560950.032.7371675
ATCGGAA558050.032.695212
Produced by FastQC (version 0.11.5)