FastQCFastQC Report
Mon 23 Dec 2019
H2CVWAFX2_n01_AH7797-3h-34C-input-spike.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVWAFX2_n01_AH7797-3h-34C-input-spike.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9414615
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC507600.5391617182433907TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA261550.047.775439
AGAGCAC275050.045.5516328
TCGGAAG318600.039.159733
GATCGGA316900.038.792111
CGGAAGA325400.038.5894054
GAAGAGC334900.037.4803626
AAGAGCA343250.036.7648967
ATCGGAA340350.036.7276152
GGAAGAG356800.035.339655
AAGGGGG123850.035.13346572-73
CATCTCG104450.034.71795338-39
CAGTCAC183650.033.9825226-27
CCAGATC180250.032.97701632-33
GTATGCC99050.032.82360544-45
CCAGTCA189950.032.79292726-27
TCCAGTC190900.032.6415724-25
AGTCACC189450.032.62920828-29
TGCCGTC84000.031.83539848-49
GCCGTCT72800.031.7418848-49
TATGCCG99900.031.47540746-47
Produced by FastQC (version 0.11.5)