FastQCFastQC Report
Mon 23 Dec 2019
H2CVWAFX2_n01_AH7797-3h-34C-Top2-spikerep.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVWAFX2_n01_AH7797-3h-34C-Top2-spikerep.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8386841
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC243910.29082463826367994TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA95100.048.1952179
AGAGCAC102100.044.7516488
GATCGGA105700.042.5282861
TCGGAAG112150.040.754243
ATCGGAA125950.036.17662
CGGAAGA133050.034.6378754
ACTTAGG65650.033.78700332-33
CTTAGGC68350.032.38283532-33
GAAGAGC150650.030.7173826
TAGGCAT74600.028.74676734-35
GAACTCC80300.028.68722320-21
ACGTCTG79650.028.68246314-15
CACTTAG78200.028.60761530-31
GGAAGAG163750.028.056895
TCCAGTC81650.028.03822924-25
CAGTCAC82600.027.74483726-27
CGTCTGA82200.027.73514716-17
AAGAGCA167250.027.6895797
GCACACG82700.027.68207610-11
TTAGGCA79850.027.39206534-35
Produced by FastQC (version 0.11.5)