FastQCFastQC Report
Mon 23 Dec 2019
H2CVWAFX2_n01_AH7797-3h-34C-Top2-spike.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVWAFX2_n01_AH7797-3h-34C-Top2-spike.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9585422
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2432652.5378642693039493TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA388100.073.5140159
AGAGCAC393000.072.658248
GATCGGA413900.067.913081
TCGGAAG419650.067.693163
ATCGGAA436800.065.1893162
CGGAAGA438400.065.0255664
GAAGAGC449700.063.4444436
AAGAGCA464450.061.542977
GGAAGAG466150.061.2361265
GTATGCC321750.042.73680544-45
GCCGTCT316500.042.56834848-49
CGATCTC289350.042.50716838-39
GATCTCG291350.042.1827738-39
CACGATC291250.042.1157136-37
CGTATGC328600.042.0988744-45
TGCCGTC324000.042.08140648-49
CATCACG330750.041.68802332-33
TATGCCG331900.041.48752646-47
CAGTCAC342650.041.3556526-27
TCGTATG333100.041.15183342-43
Produced by FastQC (version 0.11.5)