FastQCFastQC Report
Mon 23 Dec 2019
H2CVWAFX2_n01_AH10456-3h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVWAFX2_n01_AH10456-3h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10231476
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT1087761.0631506148282026TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTAT111300.1087819587320539TruSeq Adapter, Index 14 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA268950.059.495349
AGAGCAC279100.057.4851158
GATCGGA299350.052.87891
TCGGAAG309800.051.8528023
CGGAAGA325450.049.5488854
ATCGGAA327750.049.144152
GAAGAGC336050.047.9294136
AAGAGCA353450.045.5816277
GGAAGAG354650.045.3353235
CCGTATC195650.038.4782838-39
AGTTCCG202700.038.1120534-35
TATCTCG162100.037.43182440-41
GTTCCGT205200.037.41625634-35
GCCGTCT130750.037.30803750-51
GTATGCC155850.037.1353146-47
TGCCGTC146050.037.04211850-51
CGTATGC156550.036.55967346-47
AGGGGGG158300.036.548774-75
TATGCCG156050.036.4945548-49
TCCGTAT209000.036.44070436-37
Produced by FastQC (version 0.11.5)