FastQCFastQC Report
Mon 23 Dec 2019
H2CVWAFX2_n01_AH10456-3h-Red1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVWAFX2_n01_AH10456-3h-Red1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9438353
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1423881.5086106654413116TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA223300.073.7443169
AGAGCAC234750.070.289058
GATCGGA236900.068.080731
TCGGAAG242850.067.516263
CGGAAGA250750.065.57864
ATCGGAA254200.064.390542
GAAGAGC281900.058.568116
GGAAGAG295600.055.644745
AAGAGCA302600.054.5925377
AGTCACG191900.042.62147528-29
CGATCAG191300.042.1102832-33
GTCACGA193700.041.77176328-29
GCCGTCT194050.040.82911748-49
TGCCGTC197750.040.78577848-49
CACGATC198150.040.73821630-31
CGTATGC198400.040.63953844-45
GTATGCC197650.040.49335544-45
GATCTCG184000.040.29667338-39
TATGCCG200800.040.21358546-47
GCACACG204450.040.10909710-11
Produced by FastQC (version 0.11.5)