FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_SG91_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_SG91_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528585
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12640.23912899533660623No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG352.086549E-549.99756
ACGGACT700.00122535925.0011165
GACTGTA1257.8248464E-519.5971668
GGACTGT950.00720765718.4201327
AGGGGGG1351.4040092E-418.159271
CCGGAGA1450.004734006214.48340531
TGTCTGA1550.007374546513.54642743
GTAGGCT3003.4234978E-511.66718868
AGGCTAT3501.3518727E-511.00257370
TAGGCTA3702.5017964E-510.40685569
TGTAGGC3904.4655077E-59.87316967
AGTGTAG5058.0731916E-58.3179965
AGAGTGT5652.9642353E-47.43536770
AAGAGTG7105.658155E-46.40934369
GTAGGGA10103.207414E-55.890794864
AGCGTCG18501.0286385E-85.298536370
GGGAAAG8750.0068289195.20072465
GAGCGTC19302.8272552E-85.07842769
Produced by FastQC (version 0.11.5)