FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_SG162_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_SG162_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267755
Sequences flagged as poor quality0
Sequence length76
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7450.27823943530466283No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTACC200.00694294652.506354
CCTATCA750.001830088223.33177826
AACTTGG900.0052571719.4504131
ACTTGGA1107.450635E-419.09322
TGTGTTG1600.00907624313.12412523
GTAGGGA4005.1231946E-610.499368
TGTAGGG4301.2094915E-59.76861567
GTGTAGG4401.5884558E-59.546666
TAGGGAA4401.5919764E-59.54481869
AGGGAAA4502.0767851E-59.3327170
CGTGTAG4956.35572E-58.485867565
GAAAGAG4800.00375674087.29254267
TCGTGTA6107.103366E-46.886072664
GTCGTGT6107.103366E-46.886072663
CGTCGTG6259.36551E-46.72080762
GAGCGTC6803.3262945E-46.691979465
GCGTCGT6450.00133842466.512409761
AGCGTCG6600.00173505726.364400466
AGAGCGT7358.548856E-46.191219358
TCGGAAG9651.577446E-56.165392470
Produced by FastQC (version 0.11.5)