FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_SG141_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_SG141_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330033
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8850.26815500268155No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGC400.002417122234.9994727
GCCCGAA400.002417122234.9994728
AGGCTCA450.004304373631.11064124
TAGGGAC500.00720357327.99957515
GTACGCA500.00720357327.99957528
TAATCGC700.001224810524.9996261
CAGAGCC2051.2538121E-515.36562169
CTGATCA1750.001015363513.99978856
AGAGCCG2408.1436924E-411.6664970
TCAGAGC2802.3348196E-411.24982968
AATACTA3200.0088455418.74986728
AAGAGTG6201.3771278E-68.46761370
TTCAGAG4350.00143352148.04585567
GAAAGAG9551.9148665E-66.59675968
GGGAAAG8652.1245909E-56.473890367
AAAGAGT9003.7978352E-56.22212869
GGAAAGA10407.700532E-66.057600567
GTAGGGA9354.2614306E-45.61488863
TAGGGAA9354.2614306E-45.61488864
AGGGAAA10657.142123E-55.5867765
Produced by FastQC (version 0.11.5)