FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_SG138_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_SG138_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311298
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8720.28011744373558456No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG200.00694944152.49590720
AGTCGTG400.00240624335.031041
GGTACGA500.007193863428.00681529
GTAGGAT2902.4155132E-512.06802469
TAGGATA3451.1522407E-511.15854970
TGTAGGA3903.791667E-610.76839168
AGTGTAG4301.0570766E-610.58056969
GAGTGTA5653.6061701E-68.67188970
TTGCGGT3700.00300722788.51284955
AGAGTGT6754.633557E-67.777170769
AAGAGTG7803.5410114E-56.73024470
GTGTAGG16201.8189894E-126.697007767
AAAGAGT9258.219955E-66.4319369
GAAAGAG10458.321895E-66.02823866
GGGAAAG9356.616807E-55.988837264
TAGGGAA11254.33294E-65.910650370
GTAGGGA11607.27734E-65.732311269
CGTGTAG13706.282553E-75.619999470
TGTAGGG12251.8179035E-55.428148368
GGAAAGA11452.1237286E-45.19610165
Produced by FastQC (version 0.11.5)