FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_SG137_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_SG137_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242660
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32760.2636280237554922No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGGC657.9550245E-426.92578566
CGGACTG1300.00229247816.152226
TAAGGCC1500.00593321614.00084632
GTGCCTC9700.011.54708970
TGTGCCT10700.011.12127269
GTGTGCC13050.09.65497868
ACGGACT3700.00301759648.5136845
TGCCTCT8901.0550139E-98.25892570
AGTGTGC15450.08.1561667
CTATGTG5401.7661793E-47.7785665
GCCTCTA6957.073564E-67.554412470
TGTGTAG5300.00123426697.264004768
CCTCTAT7602.4893932E-56.907756369
GAGTGTG18500.06.433079766
CTCTATG7752.3819364E-46.32321767
GTGTAGA6250.00696587246.159876369
AAGAGTG26300.05.988954570
AGAGTGT21900.05.754003565
GGGAAAG32200.05.326622566
AAAGAGT34300.05.101938269
Produced by FastQC (version 0.11.5)