FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_NAC_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_NAC_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031495
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49680.24454896517097013No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG2450.027.1420466
GGACTGT4050.017.2825837
CAGTCCG2108.5930515E-716.66616827
GACTGTA4900.014.2842338
ACTGTAG4750.013.99854859
CTGTAGA7000.011.499372510
ACAGTCC3401.227299E-410.29380926
TCGGACT3106.006805E-410.1614885
CCGGACT2800.002955050710.0001945
CTACAGT5452.1726602E-58.34837424
TACAGTC4000.0060975067.87476525
AGGGGGG6552.6180198E-57.4841931
TCGTGTT8906.0653656E-77.079137370
TCTACAG7158.3613406E-56.85311123
TGTTAAG23700.06.64586269
GTAGAAG11451.3549288E-76.418864312
GTTAAGA26100.06.302959468
AGTGTTA28350.06.049797565
GTGTTAA27100.05.812206770
AGATTAG17852.5560439E-85.294350669
Produced by FastQC (version 0.11.5)