FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_LBD10_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_LBD10_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461246
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10750.2330643517775764No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCTT1807.119887E-515.5596773
ATAGTGT3050.00597012239.18076970
TGTAGGC3950.0054264857.97506670
AATGATG3950.00543177647.97420160
AGTGTAG6704.181884E-67.836197470
GAGTGTA7301.4056688E-57.19212669
AGAGTGT8351.6641425E-67.126090569
AAGAGTG9752.7021142E-66.461848368
GTAGGGA13002.288889E-75.923361368
TAGGGAA12851.1547581E-65.72011969
AGGGAAA14055.9499125E-65.23156870
TGTAGGG14802.767567E-65.203516567
GTGTAGG19607.517883E-95.178819770
AAAGAGT12551.5399189E-45.02070667
AAGAGCG29300.05.017305470
Produced by FastQC (version 0.11.5)