FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_HDL_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_HDL_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786183
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43250.242136444026172No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGG950.007215774618.41943713
CGGACTG1503.1617997E-416.332366
GCCGTGT4600.015.21818870
CCGTGGT1900.001892351112.895411570
AGCCGTG7450.011.74492469
GAGCCGT9050.010.05521368
CCGTGTA3550.00207484758.87320769
AGAGCCG10800.08.75070467
TTAGCCG4000.00610299837.873869411
ACGGACT5753.6514312E-47.3043225
GTTCAGA17450.07.22100970
TCGTGGA6005.9457123E-46.999974769
GTGTTCA18900.06.851827669
CAGAGCC10251.2389683E-76.82981866
TGTTCAG18200.06.73074569
CCGATCT11752.193774E-76.2566984
AGAGTGT28600.06.241585770
TCAGAGC13701.6121703E-86.13170870
TGTAGGG53150.05.926933370
GAGTGTT26050.05.77765470
Produced by FastQC (version 0.11.5)