FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_HB26_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_HB26_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13642116
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG212210.15555504732550288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTACA84250.015.99460368
TGGCTAC85850.015.657001567
GCTACAC88150.015.24725169
CTACACC91350.014.4458370
TTATCAC106200.012.9251891
CGATCTT142700.012.6374251
TGTGCCG21250.012.51884870
TTATCAT272800.012.438121
ACGGACT26900.012.09944859
TGATCTC108100.012.0177611
ATCCGCG15200.011.9761063
TAATCTT235400.011.7218221
CAAAGCC138950.011.71160842
TGATCCT114000.011.7029531
GGACTTT130950.011.51992461
CGCGCAC3651.7304246E-611.5053815
TTATCAA273100.011.4756331
CAATCCT90250.011.2519071
TTATCGT92650.011.2251
CGGACTT30150.011.14338760
Produced by FastQC (version 0.11.5)