FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_HALO-CCA1_eGST-LHY1_col_B_l-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_HALO-CCA1_eGST-LHY1_col_B_l-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274581
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10150.36965412756163024No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG308.358273E-658.3391046
TTGCGGA200.006948717452.49562520
GTCCGCC350.001255738139.9893830
GGTCGCC500.007197479328.00277156
CAGTCCG500.00720388627.9976727
GGACTGT657.939233E-426.9257377
ACAGTCC700.001224771124.99791726
CGAGTGT750.001830381223.33138826
GTGGTCG800.002660498321.87716354
GACTGTA800.00266331521.8731778
GGTGGTC900.00526382619.44636753
ACTGTAG1200.001338292817.4985439
GTAGAAG1503.1501788E-416.33197212
CTGTAGA1600.00907799613.12390610
TGTAGAA2050.003337371411.95022411
AAGATTA5057.330088E-47.62312769
TGTTAAG6057.917113E-57.52003568
GGGAAAG11951.07320375E-107.321566668
GAAAGAG12604.7293724E-117.222936670
GTTAAGA6401.5863297E-47.10878369
Produced by FastQC (version 0.11.5)