FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_HALO-CCA1_SBP-LHY1_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_HALO-CCA1_SBP-LHY1_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8156005
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG109410.13414655827209523No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG8100.021.6034246
GGTCGCC6600.020.68113156
CGCCGTA7400.018.91724459
GCCGTAT9400.017.8707660
TGGTCGC9050.014.69564355
GGACTGT12050.013.940597
CAGTCCG9550.011.72822127
CCGTATC12450.011.52607461
GTAGGCG7951.8189894E-1210.12656670
GTCGCCG13600.010.03655357
GACTGTA17650.09.3186038
TCGGACT11650.09.0124655
TAGTCCG8758.185452E-118.79873611
TCGCCGT15850.08.61180758
CGGACTA9353.6379788E-118.6090226
CGTATCA17600.08.352262
ACAGTCC13000.08.34643926
ACTGTAG19900.08.0891419
GTGGTCG16300.07.94437554
TGTAGGC70850.07.706874469
Produced by FastQC (version 0.11.5)