FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_DUO3_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_DUO3_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005886
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31930.3174315976164297No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGGT800.00266757721.87406726
TTCGCCG2701.6707339E-411.6673370
GTCGTGG5301.5280293E-69.24580970
GCCTGTG3050.0059799799.18084970
CGGAAGC3450.00159770529.13095470
AGTGTCG3500.00182135229.00051170
AGGGGGG4804.2499957E-48.0260781
GTTTAGC3950.00543733837.975136356
CTTCGCC5006.632515E-47.699671368
GGGTAAA4100.0075911427.68374543
AGTGTCT10302.2810127E-97.4754169
GAGTGTC11403.0377123E-107.36810768
TCTTCGC5700.00265875856.7547770
AAGAGTG18700.06.73835170
GTCTTCG6650.00190408466.316462567
GGGAAAG23250.05.871593566
AGAGTGT15606.85759E-95.83308568
AAAGAGT24200.05.784877369
TAGGGAA23401.8189894E-125.534502570
GGAAAGA25450.05.36402967
Produced by FastQC (version 0.11.5)