FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_DUO1_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_DUO1_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6576996
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCAACCTTCTTCTTGCTTCTCAAAGCTTTCATGGTGTAGCCAAAGTCC100700.15310941347691256No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90670.13785929016833826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAACC91300.029.2160724
CCGCAAC119600.029.1238883
ACCGCAA147700.028.30162
CCCGATC46050.027.5856069
CAACCCG44550.027.4167696
AACCGCA197600.027.3969521
GTAGTGT84700.026.40577728
GCAACCC52250.026.1906055
GCCTAAG98500.025.5501822
AGTAGTG88400.025.30055627
AGCCTAA101750.025.1812921
CCTAAGT97700.025.04152723
CGTGCGC2800.025.00039562
AAGCCTA103300.024.93652220
ACCCGAT49850.024.5701858
AGGCGTA38600.024.4813220
GACTCAT124850.024.44571962
ATGGACT95200.024.19174269
GCGTAAG39100.024.0811322
CGATCTT67300.024.07620211
Produced by FastQC (version 0.11.5)