FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_DAZ2_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_DAZ2_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9911500
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG262070.2644100287544771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG21050.012.47152770
TGTGCCG16600.08.64542370
GTGTGCC38400.08.4773970
AGAGCGG42400.08.338078570
TCGTGTT67500.07.51925370
GTAGGGG25350.06.904027570
AGTGTGC47200.06.59982169
GTCGTGG79050.06.509174370
GAGCGTG18052.7284841E-116.011667770
GTGCCTC31450.06.00976268
CGGAAGG20605.456968E-125.77726370
AGGGGGG50500.05.54744771
GAGTGTG88950.05.469898770
GTAGGGA129750.05.17938369
TGTAGGG153700.05.16966670
GTGTAGG169150.05.111347770
Produced by FastQC (version 0.11.5)