FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_ColSPL8_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_ColSPL8_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850373
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17300.203440137445568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG2700.047.9576036
GGACTGT3150.042.2175037
CCGGACT1750.041.9953085
GACTGTA3350.035.5184178
CGGACTA2150.032.55456
GGACTAG2200.031.8146277
GCGGACT1001.26092345E-831.496485
ACTGTAG3450.031.445769
TAGTCCG1900.029.47039211
TCGGACT2000.026.2470665
CTAGTCC2400.024.78889710
GACTAGT2700.024.6268778
CAGTCCG2850.024.56443827
ACCGGAC1502.4732799E-823.3362164
CGGACTC1352.3155917E-723.3307276
CTGTAGA4700.023.08252710
CACGGAC1102.9195178E-522.2754784
ACAGTCC3350.021.94300826
AACGGAC1604.9007213E-821.8777034
TACTGCG800.002667400821.87384217
Produced by FastQC (version 0.11.5)