FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_ColSPL16_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_ColSPL16_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1564641
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34510.2205617774300942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG6450.056.9691476
GGACTGT7000.051.99147
CGGACTA2900.043.4424826
CCGGACT3400.042.2029535
GACTGTA8650.041.262228
TCGGACT3500.040.9971545
GCGGACT2400.039.372275
ACTGTAG8750.039.1910139
CTGTAGA9500.036.4653210
TAGTCCG3400.033.96280311
TGCGGAC1109.058567E-1031.8230934
CGGACTC2600.030.9574096
CAGTCCG6900.030.94284827
ACGGACT7550.030.5939035
GGACTAG3900.030.5077787
TACAGTC7550.029.6687125
CGCGGAC604.980602E-429.171174
CTAGTCC3850.029.08423810
AACGGAC3150.028.8933494
CCCGGAC1700.028.827984
Produced by FastQC (version 0.11.5)