FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_ColSPL14_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_ColSPL14_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8940913
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG207220.2317660400006129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGCG10150.019.99951753
CTAACGC10250.017.41518864
TCCCTGC13750.015.52698552
CCGACTG12850.014.97959618
CGGGCGT12750.014.549808535
GGGCGTC13050.014.48354436
CCCGGGC13300.014.21145533
GGTCCCT14200.013.80317950
CCGGGCG13850.013.39422734
CCCCGGG13700.013.28561432
CTAGGCG13750.011.70880953
CGACTGT16700.011.52634619
TGCGATG16550.011.41870644
GTCCCCC4954.3964974E-911.31361769
TCCCCGG16150.011.27014931
CGGGAGC18150.011.185492522
GTCCCTG16950.011.15017451
GACTGGG16800.011.043134
GCTAACG17900.010.95006963
ACGCAAT18000.010.69592167
Produced by FastQC (version 0.11.5)