FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_ColSPL13_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_ColSPL13_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5416201
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG103800.19164724499700067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG6850.029.1214496
GGACTGT10100.019.0573277
CAGTCCG6400.016.40627927
GCGGACT3758.62201E-1013.9997655
CGGACTA5203.6379788E-1212.7873036
ACTGTAG15200.012.2021679
TCGGACT8900.012.1908075
AGGGGGG12900.011.9441811
GACTGTA15900.011.2247768
CCGGACT7000.010.9998155
CCGGGGT2250.00670319310.88951147
CTGTAGA16450.010.8494810
ACTCCCC5805.1773895E-89.65509951
TAGTCCG6008.7500666E-89.3319711
CGGACTC7402.7388523E-88.5127426
ACGGACT14650.08.3616355
CTCCCCT6452.4570927E-68.13954852
ACAGTCC14300.08.07693726
GTGCTGC6958.241823E-78.056972542
GGACTAG8151.400931E-77.7292187
Produced by FastQC (version 0.11.5)