FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_ColSPL11_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_ColSPL11_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3237096
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71570.22109322676868404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG8450.053.842026
GCGGACT3350.043.8806155
GGACTGT10850.040.9639637
GACTGTA11850.036.324648
ACTGTAG13700.031.674939
CAGTCCG8400.030.41903127
CGGACTA4750.030.2082066
CCGGACT4650.030.107545
CGCGGAC1253.5597623E-928.004344
TAGTCCG4300.026.85715911
CTGTAGA16450.026.5924710
TCGGACT7750.026.1935585
ACAGTCC10300.024.4679226
TACAGTC10500.024.00186325
AGCGGAC2800.022.5034874
CACGGAC3500.021.0032564
CTACAGT12900.020.35041824
GTAGAAG21700.019.99753812
CGGACTC4400.019.0894416
CTAGTCC6250.019.03765510
Produced by FastQC (version 0.11.5)