FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_ColSPL10_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_ColSPL10_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3358576
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75360.2243808090095326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG4150.031.2037286
CAGTCCG3150.023.33425727
GGACTGT5750.021.9116277
TCGGACT4400.017.5006925
GACTGTA7750.015.8042528
ACTGTAG8550.013.9161929
CGGACTA3102.3894427E-713.54791456
ACAGTCC6200.012.98419226
AGGGGGG10600.012.8840081
CCGGACT2751.9844543E-411.4549995
TACAGTC8300.010.96412625
CTGTAGA11850.010.33642310
CGCCTGT7302.2191671E-109.58970670
TAGTCCG3450.0016017279.12970811
CTACAGT9701.8189894E-129.02057224
ACGGACT10951.8189894E-128.3108315
TCGCCTG8201.800072E-88.11031970
GGACTAG4450.00180503737.86466037
GCCTGTG7755.1610186E-77.678066367
TCTACAG12505.456968E-127.56007423
Produced by FastQC (version 0.11.5)