FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_C24SPL16_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_C24SPL16_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932022
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21150.22692597385040267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG4000.054.246346
GGACTGT4350.048.2726067
CGCGGAC604.9330993E-946.673544
GACTGTA4400.045.3378528
ACTGTAG4550.043.8431979
GCGGACT1600.039.3765685
AACGGAC1850.037.8434074
TCGGACT2600.037.693815
CGGACTC1800.036.9419566
TAGTCCG1600.034.9976411
ACGGACT4900.034.2870835
CTGTAGA5950.034.11534510
GGACTAG1800.033.053337
CAGTCCG4150.032.04775227
CGGACTA2050.030.7296336
CCGGACT1500.030.3345435
TACAGTC4750.030.21011225
ACAGTCC4550.029.99958826
GTAGAAG7400.027.90352412
CTACAGT5100.027.45060224
Produced by FastQC (version 0.11.5)