FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_C24SPL15m2_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_C24SPL15m2_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23475835
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG426820.18181248931081687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG61200.011.9026491
TCCGGGC16601.8189894E-126.536309747
CCCTGCG19850.06.17099453
GGCTCGC20500.06.14661527
CTAACGC23200.06.034481564
GTCCCCC12902.0076914E-75.969156369
CCGGGCG23650.05.91977448
CGTCCCC13701.0433905E-75.87603668
CGGGCGG21857.930794E-105.125933649
GGGCGGA24158.54925E-115.072527450
Produced by FastQC (version 0.11.5)