FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_C24SPL14_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_C24SPL14_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121308
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23300.2077930416977316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG3750.055.9940076
GGACTGT4100.049.5068977
GACTGTA4200.046.659598
ACTGTAG4900.039.9939359
CAGTCCG3950.035.44240627
CTGTAGA5850.032.90099310
TCGGACT3000.032.6660845
TACAGTC4850.028.86546325
CCGGACT1601.8189894E-1228.4369955
ACAGTCC5150.026.50438126
CTACAGT5350.026.16775924
CGGACTA1801.0913936E-1125.2750726
ACGGACT5000.025.1995495
GTCCGCA700.001226663424.99955429
CACGGAC1551.2878445E-924.8438074
AACGGAC2300.024.3528214
GTAGAAG7950.024.21124512
TCTACAG5950.024.11721623
CGCCTGT1604.9161827E-821.87265859
GCGGACT1304.3454256E-621.5380785
Produced by FastQC (version 0.11.5)