FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_C24SPL11_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_C24SPL11_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5218725
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG106580.20422612802935583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG6000.026.2479766
GGACTGT9300.016.557237
GACTGTA11150.012.8677318
CAGTCCG6050.012.14898927
ACTGTAG12350.011.6175369
AGGGGGG17150.010.6174311
CCGGACT5957.212293E-99.9997095
ACAGTCC7554.1836756E-119.73528226
TAGTCCG4753.826799E-69.57747511
TCGGACT7453.274181E-109.39569955
GCCGTGT8454.0017767E-119.1134770
AACGGAC7051.19398464E-88.9375364
GCGGACT4804.488676E-58.7497455
CGGACTA5704.112897E-68.5958286
AGCCGTG12350.08.21909269
TACAGTC10851.03682396E-107.7420825
CTGTAGA19100.07.511934310
CGGACTC6854.7706424E-57.1527346
CTACAGT12251.4679244E-96.857271724
ATCGGAC6400.00123772216.56350334
Produced by FastQC (version 0.11.5)