FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_Al_SPL9_col_B_l1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_Al_SPL9_col_B_l1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976584
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27730.1402925451182444No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC2153.239472E-413.02240356
TGGTCGC2500.00115120911.19926755
TCGGTGG3451.1611091E-511.15840851
CGCCTGT5058.112775E-58.3175568
GCCTGTG5151.0196341E-48.15604469
GTCTTCG11751.8189894E-128.04406370
CTCGGTG4050.00681470757.777662350
TCGCCTG5652.978144E-47.43502367
CTATGTG5000.00561899467.0018453
GAGTGTC15351.8189894E-126.841501766
CCTGTGT8209.756335E-66.830549270
TTCGCCT7251.0005723E-46.75971866
CGGTGGT6300.00103734926.66606252
ATCTCGG5300.0098929856.603842748
CTTCGCC8903.2576892E-56.293156665
AGTGTCT16557.0940587E-105.922561667
AGAGTGT20753.274181E-115.56716865
GTAGGGA30400.05.52679369
TGTAGGG33550.05.320876668
TAGGGAA31850.05.275715470
Produced by FastQC (version 0.11.5)