FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_AGL18_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_AGL18_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134276
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18130.15983764092689962No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCCG450.004309527631.10950542
CGGACTG703.4920355E-529.9984516
GTCGACT1403.2870958E-722.49982866
TCGGACT952.7815165E-422.1050975
CCGCTTG800.002667242221.87483468
TGTCGAC2705.456968E-1219.44429865
CGACCCC1501.4965404E-518.66652768
CTGAGTC950.00721269318.42010112
CGCCTGT1351.4125032E-418.1480169
GTCACGA1000.00969071617.49986863
TCGACCC1859.0313595E-515.13568867
GACCCCA1859.034756E-515.13502169
GTCGACC1901.1339645E-414.73673266
CGACTCC3453.9981387E-914.20279168
GCCTGTG1600.00909377713.1254870
TCGCCTG1600.00909642513.12490168
GAGCCCT4052.7175702E-912.96229415
TTGTCGA3801.5517799E-812.8946464
GCCCTCC7150.012.72717817
TCCGACG7450.012.68502621
Produced by FastQC (version 0.11.5)