FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n02_AGL104_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n02_AGL104_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997073
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24780.24852743981634245No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG1550.038.3870166
GGACTGT1900.029.4721437
GCGCGAT500.00720999327.9999454
GCGGACT906.1721767E-627.223535
GACTGTA2700.022.0336728
ACTGTAG2700.020.7375749
TGGCGCT850.003790464720.58819228
GCTCTCG1350.002946280915.5555226
CAGTCCG1350.00294726615.55474227
CTGTAGA4101.4551915E-1114.51070810
CCGGACT1500.005932617514.0006715
GTGACGG1550.007371173713.54903816
AACCCTG2050.003348450411.94999530
TCGGACT2100.004005009311.6672275
TACAGTC2408.171611E-411.66605725
GTAGAAG5653.0668161E-910.53042312
AGGATAG6654.129106E-1010.00098270
GGGTGTA2900.0039481659.65515269
GTAGGAT9250.09.45943968
ATGGTCT3400.00139931179.26468652
Produced by FastQC (version 0.11.5)