FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39211768
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1627920.41516108123459267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG43700.014.43003642
ATGCCGT70300.013.75060949
GTCACGA98200.012.708467529
CAGTCAC223850.012.34587827
TATGCCG89600.011.620648
CCAGTCA240600.011.41912526
GGGGTGG637700.011.14062970
GGGGGGT550250.011.02916668
CTCGTAT90500.011.02835144
TGCCGTC89250.010.90631750
GGGGGTG590950.010.72369669
TATCGCG28650.010.66431941
GCCGTCT73600.010.57399251
TCCAGTC273100.010.155642525
CACGAAT113200.010.105453531
AATCGGG62700.09.79770141
CTCCAGT286000.09.68616824
CACTCCG80400.09.66054131
TCCGGAG82250.09.64055234
TATCTCG115600.09.58796541
Produced by FastQC (version 0.11.5)