FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_SG91_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_SG91_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528585
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT5810.10991609674886726TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGCG200.006950862252.4982959
TATGCCG1100.047.72572348
ATGCCGT1450.038.6194449
TGCCGTC1450.036.20572350
CGGACTG601.2132141E-535.00886
GCCGTCT1154.0017767E-1133.47717751
CGTATGC1800.031.110146
GTATGCC2000.027.9990947
TCGTATG2250.024.88808345
AGGGGGG800.00265261121.892931
CTCGTAT2401.8189894E-1221.8742944
CCGGTAA800.00266576221.8742948
CATCTCG2750.021.63566241
ATCTCGT2700.020.74006742
CCGTCTT1709.299947E-820.58756852
CCGTCTC850.003788807420.58756841
TCTCGTA2603.6379788E-1220.19165243
CTGCTTG1651.5903242E-619.0902959
TTCCATC3551.8189894E-1216.7600238
CCATCTC3501.8189894E-1115.99948140
Produced by FastQC (version 0.11.5)