FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_SG163_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_SG163_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72937
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT2220.30437226647654825TruSeq Adapter, Index 7 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAGG150.002217049269.9958924
TGCTCGT200.00690889952.5329485
CGGACTG200.00690889952.5329486
GAGTCTC200.006927688652.49691433
GCAGTTA200.006927688652.49691423
TACGTAG200.006927688652.49691423
TGCGTGA200.006927688652.49691460
GCATACT200.006927688652.49691469
AGAGTGG400.002405344334.9979450
TCGATTA450.004283529731.10928225
CTTGTTC500.007144900528.0175726
CGTATGC906.075059E-627.22062146
TGCCGTC906.075059E-627.22062150
ATGCCGT959.245488E-625.78795849
CTCGTAT1109.957166E-725.4530544
TCTCGTA1109.957166E-725.4530543
GTATGCC1001.3761703E-524.4985647
TCGTATG1001.3761703E-524.4985645
ATCTCGT1052.0077923E-523.33196342
GCCGTCT800.002644393621.87371451
Produced by FastQC (version 0.11.5)