FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_SG162_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_SG162_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267755
Sequences flagged as poor quality0
Sequence length76
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT2760.10307930757595563TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC853.9448387E-628.82283646
TATGCCG906.1535575E-627.22156748
CATCTCG959.364499E-625.78885541
GTATGCC1001.3938181E-524.4994147
TCTCGTA1001.3938181E-524.4994143
CCATCTC1052.0334412E-523.33277340
GCCGTCT901.9238144E-423.33277151
TGCCGTC901.9238144E-423.33277150
ATCTCGT1102.9132041E-522.27219242
ATGCCGT1102.9132041E-522.27219249
TCGTATG1205.697789E-520.41617645
CTCGTAT1257.799221E-519.5995344
CCGTCTT1107.457863E-419.0904552
AGATTCC1750.001014552213.99966435
TCCATCT1800.0012550913.61078439
CTACAAT1850.001543102113.24292612
TTCCATC1900.001886150512.89442738
GATTCCA2150.00476105411.39507536
CCAGTCA3156.919659E-49.9997670
CTCCAGT3207.996305E-49.843513568
Produced by FastQC (version 0.11.5)