FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_SG141_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_SG141_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330033
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT6830.20694900206949TruSeq Adapter, Index 7 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT200.00694808352.49920711
ATGCCGT1300.040.38400349
TGCCGTC1300.040.38400350
CGTATGC1500.039.66606546
GTATGCC1500.039.66606547
TATGCCG1550.038.38651748
GCCGTCT1400.037.49942851
TCGTATG1800.033.05505845
AGGGGGG751.484228E-632.676071
CTCGTAT1650.031.817744
GAGGTCC450.004304373631.11064127
TCTCGTA1750.029.99954643
CCGTCTT1900.027.63115952
CGTCTTC2050.025.60936753
CTTCTGC2250.024.88851256
CTGCTTG2001.8189894E-1224.49962859
CATCTCG2051.8189894E-1223.90207741
ATCTCGT2103.6379788E-1223.3329842
GCTTGAA3051.8189894E-1218.36037861
CCTCATA1150.001006048318.26059340
Produced by FastQC (version 0.11.5)