FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_SG138_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_SG138_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311298
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT8060.25891589409504717TruSeq Adapter, Index 7 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCATC200.0069477452.49915742
CGACAGC350.001254458139.99935550
GCCGTCT1750.039.99935551
TATGCCG2250.038.88826848
TGCCGTC2350.038.72278250
ATGCCGT2350.037.23344449
CGTATGC2400.036.4577546
CTCGTAT2400.036.4577544
TCGTATG2500.036.39941445
GTATGCC2450.035.7137147
CATCTCG2100.034.9994441
TCTCGTA2200.033.40855443
CCGTCTT2400.029.16619952
TGCGTGA853.9473034E-628.82306760
ATCTCGT2600.028.26877642
TCCATCT2600.028.26877639
CGTCTGC500.007202984827.9995556
CTGCTTG2100.026.66623959
GCCGTCG750.001830213823.3329651
CCATCTC3150.023.33295640
Produced by FastQC (version 0.11.5)