FastQCFastQC Report
Wed 18 Dec 2019
H2CVLAFX2_n01_SG137_col_B_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2CVLAFX2_n01_SG137_col_B_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242660
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT23090.1858110826774822TruSeq Adapter, Index 7 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5100.037.05843748
GCCGTCT4950.036.76729651
CGTATGC5250.036.66628646
ATGCCGT5100.036.37217349
TGCCGTC5750.034.08660550
GTATGCC5900.033.21999447
CCGTCTT6150.029.5931952
CTCGTAT6200.029.35453644
TCGTATG6850.027.59095445
TCTCGTA6800.025.73502743
CTGCTTG4750.025.05237259
CATCTCG8150.023.18994341
AGGGGGG1854.0199666E-1022.7079491
CGTCTTC7350.021.42834953
CTTCTGC6450.020.6199456
ATCTCGT9200.020.16283642
GCGTGAA1852.2725908E-718.91872261
CCATCTC10000.018.89980340
CGCTACT1000.00969137817.49981930
TCCATCT11650.016.22300739
Produced by FastQC (version 0.11.5)